Description
Applied Biosystems 7500 Fast Real-Time PCR System
The Applied Biosystems 7500 Fast Real-Time PCR System is a powerful and efficient quantitative PCR (qPCR) platform designed to deliver rapid, accurate, and reproducible nucleic acid analysis for research and diagnostic laboratories. Engineered with advanced thermal cycling technology and a highly sensitive optical detection system, this instrument enables scientists to perform real-time PCR experiments with exceptional speed and precision.
Designed for high-performance molecular biology workflows, the 7500 Fast system supports a 96-well plate format, allowing laboratories to process multiple samples simultaneously while maintaining excellent temperature uniformity across the block. Its fast thermal cycling capability significantly reduces run times, enabling PCR amplification in as little as 30–40 minutes, which helps laboratories increase throughput and improve productivity.
The Applied Biosystems 7500 Fast features a high-resolution CCD camera and advanced optical detection technology that enables accurate fluorescence measurement throughout the PCR process. With the ability to detect up to five different fluorescent dyes in a single reaction, the instrument supports multiplex assays for simultaneous analysis of multiple genetic targets. This capability is particularly valuable for applications requiring comprehensive molecular analysis, such as gene expression profiling, pathogen detection, and SNP genotyping.
Equipped with intuitive software, the Applied Biosystems 7500 Fast Real-Time PCR System simplifies experiment setup, monitoring, and data analysis. Researchers can easily design assays, track amplification curves in real time, and generate quantitative results with minimal manual intervention. The software also supports advanced analysis tools for relative quantification, absolute quantification, and melt curve analysis.
The Applied Biosystems 7500 Fast precise temperature control and uniform heating across the thermal block ensure consistent amplification results, providing the reliability required for demanding research environments. Its broad compatibility with commonly used fluorescent chemistries—including SYBR Green and probe-based assays—gives laboratories the flexibility to run a wide variety of molecular biology experiments.
Widely used in academic research institutions, biotechnology companies, pharmaceutical laboratories, and clinical research facilities, the Applied Biosystems 7500 Fast Real-Time PCR System is an ideal solution for laboratories seeking a fast, dependable, and versatile platform for real-time PCR analysis.
By combining speed, accuracy, and user-friendly operation, the system supports critical applications such as gene expression analysis, pathogen detection, genetic variation studies, molecular diagnostics research, and drug development, making it a valuable instrument for modern molecular biology laboratories.
Key Features Applied Biosystems 7500 Fast
-
High-Speed Real-Time PCR Performance – Fast thermal cycling enables PCR runs in as little as 30–40 minutes, improving laboratory productivity.
-
96-Well Fast Block Format – Supports 96-well plates or tube strips, allowing simultaneous analysis of multiple samples for high-throughput testing.
-
Advanced Optical Detection System – Equipped with a CCD camera and halogen light source with five excitation and emission filters for sensitive fluorescence detection.
-
Multiplex Detection Capability – Enables detection of up to five targets in a single well, allowing complex assays and multi-gene analysis in one run.
-
Wide Dynamic Range – Provides 9 logs of linear dynamic range, ensuring accurate quantification across a wide concentration range.
-
High Sensitivity – Capable of detecting as little as one copy of a nucleic acid template with high confidence.
-
Fast and Standard Run Modes – Offers flexible operation with fast mode for rapid testing and standard mode for traditional assays.
-
Precise Temperature Control – Maintains high thermal accuracy and uniformity to ensure consistent and reliable PCR amplification.
-
Compatible with Multiple Fluorescent Dyes – Supports common dyes such as FAM, SYBR Green, VIC, ROX, Cy5, TAMRA, and Texas Red for various molecular biology applications.
Key Benefits Applied Biosystems 7500 Fast
-
Rapid Results – Fast thermal cycling significantly reduces PCR run time, enabling quicker analysis and higher laboratory productivity.
-
High Sensitivity and Accuracy – Detects very low levels of nucleic acids, ensuring reliable and precise quantitative results.
-
Multiplex Detection Capability – Allows simultaneous detection of multiple genetic targets in a single reaction, saving time and reagents.
-
Improved Workflow Efficiency – 96-well format supports high-throughput testing, ideal for research and diagnostic laboratories.
-
User-Friendly Software – Intuitive data acquisition and analysis software simplifies experiment setup, monitoring, and result interpretation.
-
Flexible Applications – Suitable for gene expression analysis, pathogen detection, SNP genotyping, and molecular diagnostics.
-
Consistent Thermal Performance – Precise temperature control ensures uniform amplification and reproducible results.
-
Reliable Laboratory Performance – Designed for long-term stability and dependable operation in demanding research environments.
Specification Applied Biosystems 7500 Fast
Instrument Type
-
Real-Time PCR (qPCR) System
Sample Format
-
96-well plate
-
0.1 mL tubes or tube strips
Thermal Cycling Speed
-
Fast mode PCR runs in approximately 30–40 minutes
Temperature Range
-
4°C – 100°C
Temperature Accuracy
-
±0.25°C
Temperature Uniformity
-
±0.5°C across the block
Heating/Cooling Rate
-
Up to 5°C per second (fast mode)
Optical Detection System
-
CCD camera detection system
-
Tungsten-halogen lamp excitation source
Fluorescent Dye Compatibility
-
FAM
-
SYBR Green
-
VIC
-
ROX
-
TAMRA
-
Cy5
-
Texas Red
Multiplex Capability
-
Detection of up to 5 targets per reaction
Dynamic Range
-
Up to 9 logs of linear dynamic range
Sensitivity
-
Detects as low as 1 copy of target nucleic acid
Software
-
7500 Fast System Software for experiment setup, monitoring, and data analysis
Connectivity
-
USB / PC interface
Power Requirements
-
100–240 V AC, 50/60 Hz
Dimensions
-
Approx. 34 cm × 46 cm × 49 cm (W × D × H)
Weight
-
Approximately 34 kg
Applications
-
Gene expression analysis
-
Pathogen detection
-
SNP genotyping
-
Quantitative nucleic acid analysis
-
Molecular diagnostics
-
Drug discovery and research laboratories
